Never tried this for tree sap, but it works with gum in the hair....try rubbing with peanut butter. Scrub good with both hands with the peanut butter. Take some of the spot in each hand and rub together with the peanut butter. You'll have to wash it afterwards of course. The oil in the peanut butter works wonders. If that doesn't work, try soaking the spot with Pine-Sol for awhile before washing with a good laundry soap. I prefer Tide or Purex. Good luck.
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